Prof. Dr. Kay Grünewald participates in RESIST research projects D1 and D2.
| My Research Interest in RESIST |
Our research focus in RESIST is the structural characterisation of stages in the herpesvirus assembly pathway. For this, we use cellular electron cryo microscopy and tomography guided by advanced light microscopy techniques. Studying processes directly inside the infected cell will significantly advance the field as the current understanding of early steps was mainly derived from in vitro studies of isolated particles or bulk assays. Capturing metastable assembly intermediates will allow for novel mechanistic insights. Together with other researchers in RESIST the long-term aim is to contribute to new interventions for herpesviral diseases and improving the options for combination therapies that utilize drugs acting at different stages of the viral life cycle.
Prof. Grünewald about his scientific work
Prof. Dr. Kay Grünewald – Curriculum Vitae
Current Position
Undergraduate and Postgraduate Training
Academic and Research Posts
Other Scientific Roles
Awards and Prizes
10 Selected Publications (of in total 78 research publications in peer-reviewed journals, 12 review and opinion papers)
Ferreira JL, Pražák V, Vasishtan D, Siggel M, Hentzschel F, Binder AM, Pietsch E, Kosinski J, Frischknecht F, Gilberger TW, Grünewald K (2023) Variable microtubule architecture in the malaria parasite. Nat Commun. 14(1): 1216
Kouba T, Vogel D, Thorkelsson SR, Quemin ERJ, Williams HM, Milewski M, Busch C, Günther S, Grünewald K, Rosenthal M, Cusack S (2021) Conformational changes in Lassa virus L protein associated with promoter binding and RNA synthesis activity. Nat Commun. 12(1): 7018
Silvester E, Vollmer B, Pražák V, Vasishtan D, Machala EA, Whittle C, Black S, Bath J, Turberfield AJ#, Grünewald K#, Baker LA# (2021) DNA origami signposts for identifying proteins in cell membranes by electron cryotomography. Cell 84(4): 1110-1121 (# corresponding author)
Vollmer B, Pražák V, Vasishtan D, Jefferys EE, Hernandez-Duran A, Vallbracht M, Klupp B, Mettenleiter TC, Backovic M, Rey FA, Topf M, Grünewald K (2020) The pre-fusion structure of Herpes simplex virus glycoprotein B. Science Advances 6(39): eabc1726
Moser F, Pražák V, Mordhorst V, Andrade DM, Baker LA, Hagen C, Grünewald K, Kaufmann R (2019) Cryo-SOFI enabling low-dose super-resolution correlative light and electron cryo-microscopy. PNAS 116(11): 4804-4809
Chorev DS, Baker LA, Wu D, Beilsten-Edmands V, Rouse SL, Zeev-Ben-Mordehai T, Jiko C, Samsudin F, Gerle C, Khalid S, Stewart AG, Matthews SJ, Grünewald K, Robinson CV (2018) Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry. Science 362: 829–834
Hagen C, Dent KC, Zeev-Ben-Mordehai T, Grange M, Bosse JB, Whittle C, Klupp BG, Siebert CA, Vasishtan D, Bäuerlein FJB, Cheleski J, Werner S, Guttmann P, Rehbein S, Henzler K, Demmerle J, Adler B, Koszinowski U, Schermelleh L, Schneider G, Enquist LW, Plitzko JP, Mettenleiter TC#, Grünewald K# (2015) Structural Basis of Vesicle Formation at the Inner Nuclear Membrane. Cell 163: 1692–1701 (# corresponding author)
Kaufmann R, Schellenberger P, Seiradake E, Dobbie IM, Jones EY, Davis I, Hagen C, Grünewald K (2014) Super-Resolution Microscopy Using Standard Fluorescent Proteins in Intact Cells under Cryo-Conditions. Nano Letters 4(7): 4171-4175
Maurer UE, Sodeik B, Grünewald K (2008) Native 3D intermediates of membrane fusion in herpes simplex virus 1 entry. PNAS 105(30): 10559-10564
Grünewald K, Desai P, Winkler DC, Heymann JB, Belnap DM, Baumeister W, Steven AC (2003) Threedimensional structure of Herpes simplex virus from cryo-electron tomography. Science 302:1396-1398
