Prof. Dr. Daniel Depledge participates in RESIST research projects of area D.

My Research Interest in RESIST

My research interests focus on understanding how human herpesviruses subvert cellular processes to enhance their own survival and spread. By integrating computational biology, molecular biology, and genomics/transcriptomics approaches to diverse viral model systems, my lab group identifies and characterizes key host and viral gene products that govern herpesvirus latency and reactivation in diverse cell types. In addition, we explore the role of epitranscriptomic modifications on host and viral RNAs in regulating the outcome of infections.

Prof. Dr. Depledge about his scientific work

This Video was recorded in 2021.

Prof. Dr. Depledge – Curriculum Vitae

Current Position

  • Since 6/2021 Associate (W2) Professor of Systems Virology, Institute for Virology, MHH

Undergraduate and Postgraduate Training

  • 2000 – 2003 BSc (Hons), Biological Sciences, University of Exeter, UK

  • 2003 – 2004 MSc, Bioinformatics, University of Exeter, UK

  • 2005 – 2009 PhD, Molecular Biology, University of York, UK

Academic and Research Posts

  • 2009 – 2010 Biocurator, Wellcome Trust Sanger Institute, UK

  • 2010 – 2014 Post-doctoral Research Associate, University College London, UK

  • 2014 – 2017 MRF New Investigator Fellow, University College London, UK

  • 2017 – 2018 Post-doctoral Research Associate, NYU School of Medicine, USA

  • 2019 – 2021 Research Assistant Professor, NYU School of Medicine, USA

  • Since 2021 German Center for Infection Research (DZIF) (W2) Associate Professor, Hannover Medical School, Germany

Other Scientific Roles

  • Since 2018 ICTV Herpesvirales Study Group: member

  • Since 2019 European Virus Bioinformatics Centre: member

  • Since 2020 Member, American Society for Microbiology

  • 2022 – 2025 Editorial Board Member, Journal of Virology

Awards and Prizes

  • 2014 Medical Research Foundation (MRF) New Investigator Award

  • 2017 Daiwa Foundation Small Grant (for knowledge transfer and the establishment of a new Anglo-Japanese collaboration)

  • 2017 UCL Robin Weiss Fellowship

  • 2024 Priscilla Schaffer Memorial Lecture Award

Recommended Links

For further information about Prof. Dr. Depledge’s scientific work please check the following links:

    10 Selected Publications

    Ouwendijk WJD, Roychoudhury P, Cunningham AL, Jerome KR, Koelle DM, Kinchington PR, Mohr I, Wilson AC, Verjans GGMGM, Depledge DP. Reanalysis of single-cell RNA sequencing data does not support herpes simplex virus 1 latency in non-neuronal ganglionic cells in mice. J Virol. 2024. doi: 10.1128/jvi.01858-23

    Abebe JS, Price AM, Hayer KE, Mohr I, Weitzman MD, Wilson AC, Depledge DP. DRUMMER-Rapid detection of RNA modifications through comparative nanopore sequencing. Bioinformatics. 2022 doi: 10.1093/bioinformatics/btac274 

    Hu HL, Srinivas KP, Wang S, Chao MV, Lionnet T, Mohr I, Wilson AC, Depledge DP, Huang TT. Single-cell transcriptomics identifies Gadd45b as a regulator of herpesvirus-reactivating neurons. EMBO Rep. 2022 doi: 10.15252/embr.202153543

    Ouwendijk WJD*, Depledge DP*, Rajbhandari L, Lenac-Rovis T, Jonjic S, Breuer J, Venkatesan A, Verjans GMGM, Sadaoka T (2020) Expression of varicella-zoster virus VLT-ORF63 fusion transcript induces broad viral gene expression during reactivation from neuronal latency. Nat Commun 11(1):6324. doi: 10.1038/s41467-020-20031-4. *joint first authors 

    Price AM, Hayer EK, McIntyre ABR, Gokhale NS, Abebe JS, Della Fera AN, Mason CE, Horner SM, Wilson AC, Depledge DP, Weitzman MD (2020) Direct RNA sequencing reveals m6A modifications on adenovirus RNA are necessary for efficient splicing. Nat Commun. 11(1):6016. doi: 10.1038/s41467-020-19787-6.

    Braspenning SE, Sadaoka T, Breuer J, Verjans GMGM, Ouwendijk WJD, Depledge DP (2020) Decoding the architecture of the varicella-zoster virus transcriptome. mBio. 11(5):e01568-20. doi: 10.1128/mBio.01568-20.

    Depledge DP, Puthankalam SP, Sadaoaka T, Beady D, Mori Y, Placantonakis D, Mohr I, Wilson AC (2019) Native RNA sequencing on nanopore arrays redefines the transcriptional complexity of a viral pathogen. Nat Commun. 10(1):754. doi: 10.1038/s41467-019-08734-9.

    Depledge DP*, Ouwendijk WJD*, Sadaoka T*, Braspenning S, Mori Y, Cohrs R, Verjans GMGM#, Breuer J# (2018) A varicella-zoster virus latency transcript represses viral regulatory gene 61 expression. Nat Commun. 9(1):1167. doi: 10.1038/s41467-018-03569-2. *joint first authors #joint senior authors

    Depledge DP, Yamanishi K, Gomi Y, Gershon A, Breuer J (2016) Deep-sequencing of distinct preparations of the live-attenuated VZV vaccine reveals a conserved core of attenuating SNPs. J Virol. 2016. 90(19):8698-704. doi: 10.1128/JVI.00998-16. [Article of significant interest selected by JVI Editors].

    Depledge DP, Kundu S, Jensen NJ, Gray ER, Jones M, Steinberg S, Gershon A, Kinchington PR, Schmid DS, Balloux F, Nichols RA, Breuer J (2014) Deep sequencing of viral genomes provides insight into the evolution and pathogenesis of varicella zoster virus and its vaccine in humans. Mol Biol Evol. 31(2):397-409. doi: 10.1093/molbev/mst210.

    Contact

      Prof. Dr. Daniel Depledge
       Institute for Virology
    Hannover Medical School
      Carl-Neuberg-Str. 1
    30625 Hannover
      +49 511 532 – 4310
    Depledge.Daniel@
    mh-hannover.de