My Research Interest in RESIST |
My research interests focus on understanding how human herpesviruses subvert cellular processes to enhance their own survival and spread. By integrating computational biology, molecular biology, and genomics/transcriptomics approaches to diverse viral model systems, my lab group identifies and characterizes key host and viral gene products that govern herpesvirus latency and reactivation in diverse cell types. In addition, we explore the role of epitranscriptomic modifications on host and viral RNAs in regulating the outcome of infections.
Prof. Dr. Depledge about his scientific work
Prof. Dr. Depledge – Curriculum Vitae
Current Position
Undergraduate and Postgraduate Training
Academic and Research Posts
Other Scientific Roles
Awards and Prizes

10 Selected Publications
Ouwendijk WJD, Roychoudhury P, Cunningham AL, Jerome KR, Koelle DM, Kinchington PR, Mohr I, Wilson AC, Verjans GGMGM, Depledge DP. Reanalysis of single-cell RNA sequencing data does not support herpes simplex virus 1 latency in non-neuronal ganglionic cells in mice. J Virol. 2024. doi: 10.1128/jvi.01858-23
Abebe JS, Price AM, Hayer KE, Mohr I, Weitzman MD, Wilson AC, Depledge DP. DRUMMER-Rapid detection of RNA modifications through comparative nanopore sequencing. Bioinformatics. 2022 doi: 10.1093/bioinformatics/btac274
Hu HL, Srinivas KP, Wang S, Chao MV, Lionnet T, Mohr I, Wilson AC, Depledge DP, Huang TT. Single-cell transcriptomics identifies Gadd45b as a regulator of herpesvirus-reactivating neurons. EMBO Rep. 2022 doi: 10.15252/embr.202153543
Ouwendijk WJD*, Depledge DP*, Rajbhandari L, Lenac-Rovis T, Jonjic S, Breuer J, Venkatesan A, Verjans GMGM, Sadaoka T (2020) Expression of varicella-zoster virus VLT-ORF63 fusion transcript induces broad viral gene expression during reactivation from neuronal latency. Nat Commun 11(1):6324. doi: 10.1038/s41467-020-20031-4. *joint first authors
Price AM, Hayer EK, McIntyre ABR, Gokhale NS, Abebe JS, Della Fera AN, Mason CE, Horner SM, Wilson AC, Depledge DP, Weitzman MD (2020) Direct RNA sequencing reveals m6A modifications on adenovirus RNA are necessary for efficient splicing. Nat Commun. 11(1):6016. doi: 10.1038/s41467-020-19787-6.
Braspenning SE, Sadaoka T, Breuer J, Verjans GMGM, Ouwendijk WJD, Depledge DP (2020) Decoding the architecture of the varicella-zoster virus transcriptome. mBio. 11(5):e01568-20. doi: 10.1128/mBio.01568-20.
Depledge DP, Puthankalam SP, Sadaoaka T, Beady D, Mori Y, Placantonakis D, Mohr I, Wilson AC (2019) Native RNA sequencing on nanopore arrays redefines the transcriptional complexity of a viral pathogen. Nat Commun. 10(1):754. doi: 10.1038/s41467-019-08734-9.
Depledge DP*, Ouwendijk WJD*, Sadaoka T*, Braspenning S, Mori Y, Cohrs R, Verjans GMGM#, Breuer J# (2018) A varicella-zoster virus latency transcript represses viral regulatory gene 61 expression. Nat Commun. 9(1):1167. doi: 10.1038/s41467-018-03569-2. *joint first authors #joint senior authors
Depledge DP, Yamanishi K, Gomi Y, Gershon A, Breuer J (2016) Deep-sequencing of distinct preparations of the live-attenuated VZV vaccine reveals a conserved core of attenuating SNPs. J Virol. 2016. 90(19):8698-704. doi: 10.1128/JVI.00998-16. [Article of significant interest selected by JVI Editors].
Depledge DP, Kundu S, Jensen NJ, Gray ER, Jones M, Steinberg S, Gershon A, Kinchington PR, Schmid DS, Balloux F, Nichols RA, Breuer J (2014) Deep sequencing of viral genomes provides insight into the evolution and pathogenesis of varicella zoster virus and its vaccine in humans. Mol Biol Evol. 31(2):397-409. doi: 10.1093/molbev/mst210.
