{"id":21474,"date":"2022-03-15T01:10:25","date_gmt":"2022-03-15T00:10:25","guid":{"rendered":"https:\/\/www.resist-cluster.de\/?page_id=21474"},"modified":"2024-12-09T11:46:31","modified_gmt":"2024-12-09T10:46:31","slug":"project-d3","status":"publish","type":"page","link":"https:\/\/www.resist-cluster.de\/en\/research\/project-area-d\/project-d3\/","title":{"rendered":"Project D3"},"content":{"rendered":"

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How do viruses assemble at the single-particle level?<\/h1><\/div>

Prof. Bosse<\/a><\/p><\/h2><\/div>

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What is this research project about?<\/h1><\/div>
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Single-particle Tracking of HCMV envelopment.<\/p>\n<\/div>

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What is this research project about?<\/h1><\/div>

Human Cytomegalovirus (HCMV) is a ubiquitous betaherpesvirus of high clinical importance that establishes lifelong latent infection in humans. It is the leading cause of congenital disabilities in the developed world and a significant cause of disease in immunocompromised patients, such as transplant, AIDS, or cancer patients. It has been ranked high priority for vaccine development for over 20 years. Still, despite continuing efforts, no licensed vaccine exists so far, and antiviral therapy currently is the only treatment option, with the development of viral resistance being a significant concern.<\/p>\n<\/div>

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What\u2019s the current status?<\/h1><\/div>
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HCMV assembles in a complex order of consecutive morphogenesis events that are poorly understood at the spatiotemporal level. Highly resolved data is needed to determine how viruses assemble at the single-particle level and identify potential bottlenecks in these processes as specific pharmacological target structures.<\/p>\n<\/div>

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Light and electron microscopy of an HCMV infected cell with multiviral bodies.
\n\u00a9Flomm et al. In BioRxiv<\/a><\/p>\n<\/div>

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What are the project goals?<\/h1><\/div>

Our research aims to illuminate the spatiotemporal regulation of HCMV morphogenesis at the single-particle level. We use correlative three-dimensional live-cell microscopy electron microscopy to quantify the kinetics and dynamics of viral macromolecular complexes in living cells at the single-particle level and create computational pipelines to analyze large image datasets. Using these tools, we aim to define novel targets for inhibiting HCMV.<\/p>\n<\/div><\/div><\/div>

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How do we get there?<\/h1><\/div>
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Together with our colleagues at the MHH and the Center for Structural Systems Biology (CSSB) in Hamburg, we combine molecular virology with state-of-the-art light and electron microscopy tools, as well as large structural prediction databases that we have created using the high-performance computing centers available at the CSSB, to analyze how viruses assemble at the single-particle level.<\/p>\n<\/div>

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Clustering of herpesvirus structural groups<\/a><\/p>\n<\/div>

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Projectleaders<\/h1><\/div>

Project title: Spatiotemporal orchestration of HCMV morphogenesis<\/h4><\/div>
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Prof. Dr. Jens Bosse<\/h3><\/div>

Project: D3<\/a><\/h3><\/div>
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CV & VIDEO<\/span><\/a><\/div>
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Project D3 Publications<\/h1><\/div>

Publications 2024<\/strong><\/p>\n

A proteome-wide structural systems approach reveals insights into protein families of all human herpesviruses.<\/strong><\/a> Soh, T.K., Ognibene, S., Sanders, S. et al. Nat Commun 15, 10230 (2024)<\/p>\n

Molecular plasticity of herpesvirus nuclear egress analysed in situ.<\/strong> <\/a>Pra\u017e\u00e1k V, Mironova Y, Vasishtan D, Hagen C, Laugks U, Jensen Y, Sanders S, Heumann JM, Bosse JB, Klupp BG, Mettenleiter TC, Grange M, Gr\u00fcnewald K. Nat Microbiol. 2024 Jul;9(7):1842-1855.<\/p>\n

Publications 2023<\/strong><\/p>\n

A TriPPPro-Nucleotide Reporter with Optimized Cell-Permeable Dyes for Metabolic Labeling of Cellular and Viral DNA in Living Cells.<\/strong><\/a>\u00a0 Sterrenberg VT, Stalling D, Knaack JIH, Soh TK, Bosse JB, Meier C. Angew Chem Int Ed Engl. 2023 Sep 18;62(38):e202308271.<\/p>\n

HSV-1 exploits host heterochromatin for nuclear egress.<\/strong><\/a> Lewis HC, Kelnhofer-Millevolte LE, Brinkley MR, Arbach HE, Arnold EA, Sanders S, Bosse JB, Ramachandran S, Avgousti DC. J Cell Biol. 2023 Sep 4;222(9):e202304106.<\/p>\n

Spatially resolved protein map of intact human cytomegalovirus virions.<\/strong> <\/a>Bogdanow B, Gruska I, M\u00fchlberg L, Protze J, Hohensee S, Vetter B, Bosse JB, Lehmann M, Sadeghi M, Wiebusch L, Liu F. Nat Microbiol. 2023 Aug 7.<\/p>\n

From the beginnings to multidimensional light and electron microscopy of virus morphogenesis.<\/strong><\/a>\u00a0Saskia Sanders, Yannick Jensen, Rudolph Reimer, Jens B. Bosse, Chapter Two – , Editor(s): Stefan Finke, Dmitry Ushakov, Advances in Virus Research, Academic Press, Volume 116, 2023, Pages 45-88<\/p>\n

Viral dew: Phase separation and the formation of viral replication compartments<\/strong>.<\/a> Bosse JB, Brune W. Viral dew. PLoS Pathog. 2023 Feb 16;19(2):e1011145.<\/p>\n

A validated protocol to UV-inactivate SARS-CoV-2 and herpesvirus-infected cells.<\/strong><\/a> PLoS One. 2023 May 10;18(5):e0274065. Soh TK, Pfefferle S, Wurr S, von Possel R, Oestereich L, Rieger T, Uetrecht C, Rosenthal M, Bosse JB.<\/p>\n

Publications 2022<\/strong><\/p>\n

Intermittent bulk release of human cytomegalovirus.<\/a><\/strong> Flomm FJ, Soh TK, Schneider C, Wedemann L, Britt HM, Thalassinos K, Pfitzner S, Reimer R, Gr\u00fcnewald K, Bosse JB. PLoS Pathog. 2022 Aug 4<\/p>\n

The unconventional way out \u2013 Egress of HCMV through multiviral bodies.<\/strong><\/a>\u00a0Wedemann L, Flomm FJ, Bosse JB. Mol Microbiol.<\/p>\n

Herpesvirus Replication Compartments: Dynamic Biomolecular Condensates?<\/strong> <\/a>Enrico Caragliano, Wolfram Brune, Jens B. Bosse, Viruses<\/p>\n

Human cytomegalovirus forms phase-separated compartments at viral genomes to facilitate viral replication.<\/a> <\/strong>Caragliano E, Bonazza S, Frascaroli G, Tang J, Soh TK,Gr\u00fcnewald K, Bosse JB*, Brune W* (*co-corresponding authors, equal contribution) Cell Reports<\/p>\n

A Unique Role of the Human Cytomegalovirus Small Capsid Protein in Capsid Assembly.<\/strong>\u00a0<\/a>Borst EM, Harmening S, Sanders S, Caragliano E, Wagner K, Lenac Rovi\u0161 T, Jonji\u0107 S, Bosse JB, Messerle M.\u00a0 mBio. 2022 Oct 26;13(5):e0100722. doi: 10.1128\/mbio.01007-22. Epub 2022 Sep 6.<\/p>\n

Publications 2021<\/strong><\/p>\n

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Infection-induced chromatin modifications facilitate translocation of herpes simplex virus capsids to the inner nuclear membrane<\/strong><\/a>, V Aho, S Salminen, S Mattola, A Gupta, F Flomm, B Sodeik, JB Bosse, Maija Vihinen-Ranta, PLoS pathogens 17 (12), e1010132<\/p>\n

KIR3DS1 directs NK cell-mediated protection against human adenovirus infections<\/a><\/strong>. Jung J, Ching W, Baumdick M, Hoffmann-Sieber H, Bosse JB, Koyro TF, Moeller K, Wegner L, Niehrs A, Russu K, Ohms M, Zhang W, Ehrhardt A, Duisters K, Spierings E, Hoelzemer A, Koerner C, Jansen S, Peine S, Koenigs I, Luetgehetmann M, Perez D, Reinshagen K, Lindemanns AA, Altfeld M, Berlderbos ME, Dobner T, Bunders M, (2021), Science Immunology, https:\/\/doi.org\/10.1126\/sciimmunol.abe2942<\/p>\n

Concatemeric Broccoli reduces mRNA stability and induces aggregates.<\/strong> <\/a>Rink MR, Baptista MAP, Flomm FJ, Hennig T, Whisnant AW, Wolf N, Seibel J, D\u00f6lken L, Bosse JB, (2021), PLOS One, doi: 10.1371\/journal.pone.0244166 Article PLOS ONE 3,2<\/p>\n

Identification of African Elephant Polyomavirus in wild elephants and the creation of a vector expressing its viral tumor antigens to transform elephant primary cells.<\/strong><\/a> Pearson VR, Bosse JB, Koyuncu OO, Scherer J, Toruno C, Robinson R, et al. (2021)\u00a0 PLoS ONE 16(2): e0244334.<\/p>\n

Human Adenovirus Type 5 Infection Leads to Nuclear Envelope Destabilization and Membrane Permeability Independently of Adenovirus Death Protein<\/strong><\/a>. Pfitzner S, Bosse JB, Hofmann-Sieber H, Flomm F, Reimer R, Dobner T, Gr\u00fcnewald K, Franken LE, International Journal of Molecular Sciences (IJMS) 2021).<\/p>\n<\/div>\n

Egress of human cytomegalovirus through multivesicular bodies.<\/strong> <\/a>Flomm F, Soh TK, Britt H, Schneider C, Reimer R, Thalassinos K, Gr\u00fcnewald K, Bosse JB. bioRxiv 2020<\/p>\n

Publications 2020<\/strong><\/p>\n

Fluorescent Protein Tagging of Adenoviral Proeins PV and PIX Reveals ‘Late Virion Accumulation Compartment’.<\/strong><\/a> Sieber H, Bosse JB, Franken LE, Gr\u00fcnewald K, Dobner T, (2020), PLOS Pathogens<\/p>\n<\/div>

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Publications of the Project D3<\/h1><\/div>
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