{"id":20083,"date":"2022-02-11T10:14:21","date_gmt":"2022-02-11T09:14:21","guid":{"rendered":"https:\/\/www.resist-cluster.de\/?page_id=20083"},"modified":"2026-01-20T08:33:51","modified_gmt":"2026-01-20T07:33:51","slug":"project-b13","status":"publish","type":"page","link":"https:\/\/www.resist-cluster.de\/en\/research\/project-area-b\/project-b13\/","title":{"rendered":"Project B13"},"content":{"rendered":"

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How can we fight lung infections?<\/h1><\/div>

Prof. Lachmann<\/a><\/p><\/h2><\/div>

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What is this research project about?<\/h1><\/div>
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Fluorescence image of macrophages from genetically modified stem cells (iPSC).
\nCopyright: Shifaa Abdin<\/p>\n<\/div>

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What is this research project about?<\/h1><\/div>

Within our research, we are focusing on infections of the lower respiratory tract such as pneumonia, which is still the most communicable disease worldwide. Our research aims to understand the onset of infectious diseases and to establish new therapeutic options based on approaches using regenerative medicine. In our research we use cells from patients, to establish new infection models, which will pave the way for cell-based immunotherapeutic approaches targeting infections of the lower respiratory tract. Our focus is primarily on the macrophages (phagocytes) of the immune system.<\/p>\n<\/div>

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What\u2019s the current status?<\/h1><\/div>
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We have been successful in establishing a plethora of different infection models for bacteria and viruses. Based on this knowledge, we could also achieve the use of newly designed immune cells to combat life-threatening pulmonary infections. As of now, we are aiming to push these findings into the clinics. In particular, we are investigating the role of macrophages (scavenger cells) in the development of and fight against infections. We are using different types of stem cells for this purpose. One of our approaches is to replace the weakened phagocytes with healthy phagocytes from the laboratory, which then offer protection against bacteria or viruses.<\/p>\n<\/div>

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Macrophages freshly derived from differentiation cultures of human stem cells (iPSC).
\nCopyright: Dr. Mania Ackermann<\/p>\n<\/div>

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What are the project goals?<\/h1><\/div>

Our goals are new immunotherapeutic approaches using either designer immune cells or other immunotherapeutic approaches to combat pulmonary infections. We are focusing primarily on bacterial infections, with the long-term goal to use established systems also for viral intruders.<\/p>\n<\/div><\/div><\/div>

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How do we get there?<\/h1><\/div>
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In order to gain insights into the development of lung diseases and to be able to derive therapies from them, we establish different disease models in the laboratory. To do this, we are working in a highly inter-disciplinary environment, in which we combine several disciplines such as infection and regenerative medicine, stem cells and immunity. We are using the framework of RESIST to combine medical scientist with clinician scientist, to pave the development of new innovations and radical new ideas to combat bacterial pneumonia.<\/p>\n

The production of iPSC immune cells in scalable systems and the modification of the cells open up new possibilities for research into new cell-based immunotherapy against various pathogens.<\/p>\n<\/div>

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The diagram shows how we produce the cells and the concepts we pursue with them.<\/p>\n<\/div>

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Projectleader Prof. Lachmann<\/h1><\/div>

Project title: Control of Airway Infections<\/h4><\/div>
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\"Nico<\/a><\/span><\/div>
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Prof. Dr. Nico Lachmann<\/h3><\/div>

Project: B13<\/a><\/h3><\/div>
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CV & VIDEO<\/span><\/a><\/div>
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Publications of the Project B13<\/h1><\/div>

Publications 2026<\/strong><\/p>\n

Commensal microflora coating endows implants with biofilm-repellent, immunomodulatory and osteogenic properties<\/strong><\/a> M. I. Rahim, S. Baseer, D. Paasch, M. Steglich, S. F. H. Waqas, N. Lachmann, et al. Acta Biomater<\/strong> 2026 Accession Number: 41519361 DOI: 10.1016\/j.actbio.2026.01.017<\/p>\n

Immune activation and response dynamics of human iPSC-derived macrophages in tuberculosis infection models<\/strong><\/a> D. Paasch, H. Schevel, A. Riehle, B. Costa, H. Toufaili, T. Gehnen, et al. Clin Transl Immunology<\/strong> 2026 Vol. 15 Issue 1 Pages e70071 Accession Number: 41541231 PMCID: PMC12800570 DOI: 10.1002\/cti2.70071<\/p>\n

Publications 2025<\/strong><\/p>\n

In vivo macrophage engineering for renal cancer therap<\/strong><\/a>y D. Bas\u00edlio-Queir\u00f3s and N. Lachmann Nat Cancer<\/strong> 2025 Accession Number: 40301654 DOI: 10.1038\/s43018-025-00912-7
\nSensory neurons shape local macrophage identity via TGF-\u03b2 signaling<\/strong><\/a> J. Kolter, C. L. D\u00f6ring, S. Sarout, S. Baasch, L. Steele, R. Alsumati, et al.Immunity<\/strong> 2025 Accession Number: 40914152 DOI: 10.1016\/j.immuni.2025.08.004<\/p>\n

Publications 2024<\/strong><\/p>\n

CAR macrophages on a fast track to solid tumor therapy.\u00a0<\/b><\/a> Abdin SM, Paasch D, Lachmann N. Nat Immunol.<\/strong> 2024 Jan;25(1):11-12. doi: 10.1038\/s41590-023-01696-7. PMID: 38168956.<\/p>\n

Pulmonary Alveolar Proteinosis and new therapeutic concepts.<\/b><\/a> Rodriguez Gonzalez C, Schevel H, Hansen G, Schwerk N, Lachmann N.
\nKlin Padiatr.<\/strong> 2024 Feb;236(2):73-79. doi: 10.1055\/a-2233-1243. Epub 2024 Jan 29. PMID: 38286410 Free PMC article.<\/p>\n

Sensor macrophages derived from human induced pluripotent stem cells to assess pyrogenic contaminations in parenteral drugs.<\/b><\/a> Abdin, S. M., F. Mansel, A. R. Hashtchin, M. Ackermann, G. Hansen, B. Becker, B. Kick, N. Pham, H. Dietz, C. Schaniel, U. Martin, I. Spreitzer, and N. Lachmann. 2024.\u00a0 Biofabrication<\/strong> 16.<\/p>\n

<\/b>Adaptation of Human iPSC-Derived Macrophages Toward an Alveolar Macrophage-Like Phenotype Post-Intra-Pulmonary Transfer into Murine Models.<\/b><\/a> Hetzel, M., I. Gensch, M. Ackermann, and N. Lachmann. 2024.\u00a0 Methods in Molecular Biology<\/strong> (Clifton, N.J.) 2713: 463-479.<\/p>\n

Human CFTR deficient iPSC-macrophages reveal impaired functional and transcriptomic response upon Pseudomonas aeruginosa infection<\/a><\/strong> C. Rodriguez Gonzalez, D. Bas\u00edlio-Queir\u00f3s, A. L. Neehus, S. Merkert, D. Tschritter, S. \u00dcnal, et al. Front Immunol<\/strong> 2024 Vol. 15 Pages 1397886 Accession Number: 39606224 PMCID: PMC11601075 DOI: 10.3389\/fimmu.2024.1397886<\/p>\n

Ultrasound-triggered drug release in vivo from antibubble-loaded macrophages<\/strong><\/a> O. Desai, M. K\u00f6ster, D. Kloos, N. Lachmann, H. Hauser, A. Poortinga, et al.J Control Release<\/strong> 2024 Accession Number: 39653149 DOI: 10.1016\/j.jconrel.2024.12.007<\/p>\n

Tuberculosis in otherwise healthy adults with inherited TNF deficiency<\/strong><\/a> A. A. Arias, A. L. Neehus, M. Ogishi, V. Meynier, A. Krebs, T. Lazarov, et al. Nature<\/strong> 2024 Vol. 633 Issue 8029 Pages 417-425 Accession Number: 39198650 PMCID: PMC11390478 DOI: 10.1038\/s41586-024-07866-3<\/p>\n

Standardized generation of human iPSC-derived hematopoietic organoids and macrophages utilizing a benchtop bioreactor platform under fully defined conditions<\/strong><\/a>\u00a0 M. Ackermann, F. Saleh, S. M. Abdin, A. Rafiei Hashtchin, I. Gensch, J. Golgath, et al.Stem Cell Res Ther<\/strong> 2024 Vol. 15 Issue 1 Pages 171 Accession Number: 38886860 PMCID: PMC11184717 DOI: 10.1186\/s13287-024-03785-2<\/p>\n

Publications 2023<\/strong><\/p>\n

Human IRF1 governs macrophagic IFN-\u03b3 immunity to mycobacteria.<\/strong><\/a>\u00a0Rosain J, Neehus AL, Manry J, Yang R, Le Pen J, Daher W, Liu Z, Chan YH, Tahuil N, T\u00fcrel \u00d6, Bourgey M, Ogishi M, Doisne JM, Izquierdo HM, Shirasaki T, Le Voyer T, Gu\u00e9rin A, Bastard P, Moncada-V\u00e9lez M, Han JE, Khan T, Rapaport F, Hong SH, Cheung A, Haake K, Mindt BC, P\u00e9rez L, Philippot Q, Lee D, Zhang P, Rinchai D, Al Ali F, Ahmad Ata MM, Rahman M, Peel JN, Heissel S, Molina H, Kendir-Demirkol Y, Bailey R, Zhao S, Bohlen J, Mancini M, Seeleuthner Y, Roelens M, Lorenzo L, Soud\u00e9e C, Paz MEJ, Gonz\u00e1lez ML, Jeljeli M, Soulier J, Romana S, L\u2019Honneur AS, Materna M, Mart\u00ednez-Barricarte R, Pochon M, Oleaga-Quintas C, Michev A, Migaud M, L\u00e9vy R, Alyanakian MA, Rozenberg F, Croft CA, Vogt G, Emile JF, Kremer L, Ma CS, Fritz JH, Lemon SM, Spaan AN, Manel N, Abel L, MacDonald MR, Boisson-Dupuis S, Marr N, Tangye SG, Di Santo JP, Zhang Q, Zhang SY, Rice CM, B\u00e9ziat V, Lachmann N, Langlais D, Casanova JL, Gros P, Bustamante J. Cell.<\/strong> 2023 Feb 2;186(3):621-645.e33. doi: 10.1016\/j.cell.2022.12.038. PMID: 36736301; PMCID: PMC9907019.<\/p>\n

Metabolic rewiring tunes dermal macrophages in staphylococcal skin infection.<\/strong><\/a> Sci Immunol<\/strong>. Forde AJ, Kolter J, Zwicky P, Baasch S, Lohrmann F, Eckert M, Gres V, Lagies S, Gorka O, Rambold AS, Buescher JM, Kammerer B, Lachmann N, Prinz M, Gro\u00df O, Pearce EJ, Becher B, Henneke P.\u00a0 2023 Aug 18;8(86):eadg3517.<\/p>\n

Alveolar macrophages in tissue homeostasis, inflammation, and infection: evolving concepts of therapeutic targeting.<\/strong><\/a>\u00a0Malainou C, Abdin SM, Lachmann N, Matt U, Herold S. J Clin Invest.<\/strong> 2023 Oct 2;133(19):e170501. doi: 10.1172\/JCI170501. PMID: 37781922; PMCID: PMC10541196.<\/p>\n

Scalable generation of functional human iPSC-derived CAR-macrophages that efficiently eradicate CD19-positive leukemia.<\/strong><\/a>\u00a0Abdin SM, Paasch D, Kloos A, Oliveira MC, Jang MS, Ackermann M, Stamopoulou A, Mroch PJ, Falk CS, von Kaisenberg CS, Schambach A, Heuser M, Moritz T, Hansen G, Morgan M, Lachmann N.\u00a0 J Immunother Cancer.<\/strong> 2023 Dec 22;11(12):e007705. doi: 10.1136\/jitc-2023-007705. PMID: 38135346; PMCID: PMC10749073.<\/p>\n

An evaluation of an open access iPSC training course: “How to model interstitial lung disease using patient-derived iPSCs”.<\/strong><\/a> Schweikert A, Kenny S, Oglesby I, Glasgow A, de Santi C, Gensch I, Lachmann N, Desroziers T, Fletcher C, Snijders D, Nathan N, Hurley K; COST Open-ILD Group Management Committee. Stem Cell Res Ther.<\/strong> 2023 Dec 20;14(1):377. doi: 10.1186\/s13287-023-03598-9. PMID: 38124115; PMCID: PMC10734099.<\/p>\n

Publications 2022<\/strong><\/p>\n

Generation of Human iPSC from Small Volume Peripheral Blood Samples.<\/strong><\/a>\u00a0Kloos D, Lachmann N. Methods Mol Biol.<\/strong> 2022;2429:27-39. doi: 10.1007\/978-1-0716-1979-7_3. PMID: 35507153<\/p>\n

ISG15 deficiency features a complex cellular phenotype that responds to treatment with itaconate and derivatives.<\/strong><\/a>\u00a0Waqas SF, Sohail A, Nguyen AHH, Usman A, Ludwig T, Wegner A, Malik MNH, Schuchardt S, Geffers R, Winterhoff M, Merkert S, Martin U, Olmer R, Lachmann N, Pessler F. Clin Transl Med<\/strong>. 2022<\/p>\n

Targeted biallelic integration of an inducible Caspase 9 suicide gene in iPSCs for safer therapies.<\/strong>\u00a0<\/a>Wunderlich S, Haase A, Merkert S, Jahn K, Deest M, Frieling H, Glage S, Korte W, Martens A, Kirschning A, Zeug A, Ponimaskin E, G\u00f6hring G, Ackermann M, Lachmann N, Moritz T, Zweigerdt R, Martin U. Mol Ther Methods Clin Dev.<\/strong><\/p>\n

Continuous human iPSC-macrophage mass production by suspension culture in stirred tank bioreactors.<\/strong>\u00a0<\/a>Ackermann M, Rafiei Hashtchin A, Manstein F, Carvalho Oliveira M, Kempf H, Zweigerdt R, Lachmann N. Nat Protoc<\/strong>. 2022 Jan 17.<\/p>\n

In vitro systems to study inborn errors of immunity using human induced pluripotent stem cells<\/strong><\/a> E. Nikolouli, J. Reichstein, G. Hansen and N. Lachmann Frontiers in Immunology<\/strong> 2022 Vol. 13 Pages 1024935 DOI: 10.3389\/fimmu.2022.1024935<\/p>\n

Publications 2021<\/strong><\/p>\n

Beyond \u201eBig Eaters\u201c: The Versatile Role of Alveolar Macrophages in Health and Disease.<\/a><\/strong>\u00a0Hetzel M, Ackermann M, Lachmann N. Int J Mol Sci.<\/strong> 2021 Mar 24;22(7):3308. doi: 10.3390\/ijms22073308. PMID: 33804918 Free PMC article. Review.<\/p>\n

Human iPSC-derived macrophages for efficient Staphylococcus aureus clearance in a murine pulmonary infection model.<\/strong>\u00a0<\/a>Rafiei Hashtchin A, Fehlhaber B, Hetzel M, Manstein F, Stalp JL, Glage S, Abeln M, Zweigerdt R, Munder A, Viemann D, Ackermann M, Lachmann N. Blood Adv.<\/strong> 2021 Dec 14;5(23):5190-5201.<\/p>\n

CARs and beyond: tailoring macrophage-based cell therapeutics to combat solid malignancies.<\/strong>\u00a0<\/a>Abdin SM, Paasch D, Morgan M, Lachmann N. J Immunother Cancer.<\/strong> 2021 Aug;9(8):e002741. doi: 10.1136\/jitc-2021-002741. PMID: 34462325; PMCID: PMC8407221.<\/p>\n

Genetic Correction of IL-10RB Deficiency Reconstitutes Anti-Inflammatory Regulation in iPSC-Derived Macrophages.<\/strong>\u00a0<\/a>Hoffmann D, Sens J, Brennig S, Brand D, Philipp F, Vollmer Barbosa P, Kuehle J, Steinemann D, Lenz D, Buchegger T, Morgan M, Falk CS, Klein C, Lachmann N, Schambach A.\u00a0 J Pers Med.<\/strong> 2021 Mar 20;11(3):221. doi: 10.3390\/jpm11030221. PMID: 33804706; PMCID: PMC8003874.<\/p>\n

Impaired respiratory burst contributes to infections in PKC\u03b4-deficient patients<\/strong><\/a> A. L. Neehus, K. Moriya, A. Nieto-Patl\u00e1n, T. Le Voyer, R. L\u00e9vy, A. \u00d6zen, et al. J Exp Med<\/strong> 2021 Vol. 218 Issue 9 Accession Number: 34264265 PMCID: PMC8288504 DOI: 10.1084\/jem.20210501<\/p>\n

iPSC modeling of stage-specific leukemogenesis reveals BAALC as a key oncogene in severe congenital neutropenia<\/strong><\/a> B. Dannenmann, M. Klimiankou, B. Oswald, A. Solovyeva, J. Mardan, M. Nasri, et al. Cell Stem Cell<\/strong> 2021 Vol. 28 Issue 5 Pages 906-922.e6 DOI: 10.1016\/j.stem.2021.03.023<\/p>\n<\/div>

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Publications of the Project B13<\/h1><\/div>
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